To label the HIV-1 gp120 envelope glycoprotein, HEK-293T cells (American Type Culture Collection) grown to 70–80% confluence were transfected (GenePorter transfection reagent; Gene Therapy ...

A method for genetically barcoding Drosophila is developed and used to tag defined cell populations in vivo for single-cell transcriptomics experiments and to enable multiplexed behavioral analysis.

48 h after transfection, cell lines were treated with 2 μg/mL puromycin for 2 weeks and screened for cell lines stably overexpressing or silencing ALKBH5/IGF2BP2. The primer sequences are shown in ...

HRP-conjugated goat anti-mouse or anti-rabbit secondary antibodies for Western blot were purchased from Santa Cruz (USA). JetPRIME kit was obtained from Polyplus Transfection (France). Anti-pA151R ...

This valuable study presents a mouse gastruloid model that can be used to generate hematopoietic progenitors as well as leukemic cells. However, in its current form, the manuscript is inadequate ...

You have full access to this article via your institution. This intrinsic property of AAV to undergo intermolecular concatamerization had shown great potential for expanding the current packaging ...