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Then, 0.4 mM IPTG (isopropyl-D-thiogalactopyranoside) was added to induce protein expression. After 4 h incubation at 16°C, cells were harvested and resuspended in lysing buffer (10 mM Tris-Cl [pH 7.8 ...
Julian Stratenschulte via Getty Images The UM engineering team’s solution is a 20-nanometer-thick lithium borate-carbonate application to the battery. Combining this glassy material coating with ...
To solve this, the team coated the battery with a thin, glassy material made of lithium borate-carbonate—only 20 nanometers thick—which prevented the problematic chemical layer from forming.
Tris buffers actually hamper DNA electrophoresis through their high conductivity. The heat and current generated during an electrophoresis run can melt and distort the gel. Seeking a better formula, ...
These schemes put 65% of the portfolio in debt and the remaining 35% in arbitrage funds to reap the benefit of 12.5% long-term capital gains tax, as pure debt funds are taxed at your income tax ...
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