The gel is then soaked in alkaline solution to denature the DNA, and then neutralized in Tris/NaCl solution. The latter step is optional for nylon blots, which are more resistant to alkaline ...
TGEM(0.1): (0.1 refers to the NaCl concentration) 20 mM Tris-HCl (pH 7.9), 20% glycerol, 1 mM EDTA, 5 mM MgCl 2, 0.1% NP-40, 1 mM dithiothreitol (DTT), 0.2 mM phenylmethylsulfonyl fluoride (PMSF ...
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