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Embryos were fixed for 30 min at room temperature with 4% paraformaldehyde in MOPS buffer (0.1 M 3-(N-morpholino) propane sulfonic acid), adjusted to a pH value of 7.5, and washed in PBT two times.

NOTE 4: A temporary site for the online version of tcsBU is now available at (http://www.fc.up.pt/pessoas/amsantos/tcsBU). As a consequence, it takes a significant ...