In this protocol, we detail a seven-stage differentiation methodology for generating pancreatic delta cells (SC-delta cells) from human pluripotent stem cells (hPSCs). In the first step, definitive ...

Taking the soluble copper content in water quality samples as the research object, a mathematical model was established and flame atomic absorption spectrophotometry was used to analyze the ...

2. Filter magnetic microbead solution through 0.22 porous sterile filter in sterile hood and place in 4°C until needed. 3. Add 3 mL of DMEM/F12 GLUTAMAX with 10% FBS and 1% Pen/Strep, hereafter ...

Pipetting products are used for dispensing liquid of about 5–25 ml (pipettes), 1 ml (Pasteur pipettes), and low volumes from 1 µl to 1000 µl (micropipettes) from one container to the other.

Most mammals maintain their body temperature around 37°C, whereas in hibernators it can approach 0°C without triggering a thermogenic response. The remarkable plasticity of the thermoregulatory system ...