By contrast, thermal cyclers use electric heating and cooling components to drive the PCR reaction through each of its three phases: denaturation, annealing and extension. During denaturation ...

since the annealing temperature of the fluorescent dye-labeled M13(-21) primer is only 53°C, the last eight PCR cycles should be run with an annealing temperature of 53°C (the same result can be ...

PCR involves the enzymatic amplification of nucleic acid templates, and can be divided into four major steps, listed below. 1 Denaturation: Double-stranded DNA separates into single strands. Annealing ...

Invitrogen™ Platinum™ SuperFi II DNA Polymerase is a hot-start, engineered proofreading DNA polymerase, providing superior fidelity and specificity to your PCR. With >300x Taq fidelity and buffer ...

PCR amplifies DNA in a three-step process: denaturation, which melts double-stranded DNA into single strands; annealing, which lets small pieces of primer DNA bind to either side of the region of ...