DKK1 表达与 DNA 甲基化的关系 ：研究人员通过 BSAS 检测了结直肠癌细胞系和组织中 DKK1 基因的甲基化模式，发现肿瘤组织中多个位于启动子、5’UTR 和外显子 1 区域的 CpG 位点甲基化水平高于相邻正常组织。在细胞系中，DLD1 和 RKO 细胞的 DKK1 基因高甲基化，而 SW480 细胞非甲基化，且 DKK1 表达水平与 DNA 甲基化状态相关。此外，用 5 - 氮杂 - ...

Cell lysates were directly added to the bisulphite conversion protocol (see below) with no additional handling. The use of human stool samples was approved by the University of Otago Human Ethics ...

Flowering plants utilize small RNA (sRNA) molecules to guide DNA methyltransferases to genomic sequences. This RNA-directed DNA methylation (RdDM) pathway preferentially targets euchromatic ...

Alternation between morphologically distinct haploid and diploid life forms is a defining feature of most plant and algal life cycles, yet the underlying molecular mechanisms that govern these ...