Representative H&E staining of liver tissue from male WT and DKO animals ... 10,300rpm for 10 minutes at 4°C in ultracentrifuge. The nuclei pellet was resuspended in FACS buffer (1x PBS, 2.5% (v/v) ...

EDU solution (10 mM) was added and incubated for 2 hours at 37°C. Following fixation with 4% paraformaldehyde, the cells were washed, permeabilized, and subjected to fluorescent dye staining. Flow ...