This protocol describes the resolution of genomic DNA by TAFE, followed by blotting and hybridization. 1 | Cast a 1% agarose gel in 1× TAFE gel buffer (20 mM Tris-acetate (pH 8.2), 0.5 mM EDTA ...
With an E-Gel ® iBase™ Power System, separation of DNA can typically be completed in just 7 minutes, perfect for quick analysis of your DNA fragment prior to downstream applications.
In combination with EMBER™ DNA Loading Dye or EMBER™ Ultra RNA Loading Dye, this system supports RNA and DNA agarose gels between 1% and 5%, and does not require post-electrophoresis staining for fast ...
Resolution of OligoPrep (left) versus standard agarose (right). OligoPrep TM Synthetic Meltable Gel Matrix From National Diagnostics Imagine that purification of DNA or protein fragments was ...
During this week you will carry out restriction digestion of the genomic clone, in order to cut the insert (=the gene) from the plasmid DNA (=vector). Following separation of insert and vector by ...
In the current installment, [Justin] covers the basics of agarose gel electrophoresis ... like fluorescent DNA stain for visualizing the bands; we’re heartened to see that the potent carcinogen ...
For DNA analysis, 10 µL of each 50 µL elution was loaded onto a 1× TAE agarose gel. As it can be seen, the phage DNA was safely protected from the DNase I treatment by its coat protein ...