The beads were washed twice and resuspended with 25 µL of EM buffer A. 20 µL of this mixture was transferred to a 0.5 ml protein LoBind tube and mixed with 640 pmol of SPYtag-GFP nanobody and ...

KAUST Catalysis Center (KCC), Division of Physical Sciences and Engineering, King Abdullah University of Science and Technology (KAUST), Thuwal 23955, Kingdom of Saudi Arabia ...

Louis, MO). For immunoprecipitation, the dimerized GFP nanobody construct LaG-16–G4S–LaG-2 (green lobster; gift from Michael Rout), mTOR 7C10, and Normal Rabbit IgG (2729S; Cell Signaling Technology, ...

As model proteins, we chose two Nbs, one being specific for human epidermal growth factor receptor (EGFR) (Schmitz et al., 2013), while the other one was recognizing green fluorescent protein (GFP) ...

Here, we have developed and used a novel, nanobody-based toolset, the GrabFP system, to interfere with the localization of GFP-tagged proteins along the apical-basal axis in the larval wing imaginal ...