Figure 1: Retroviral backbone of pSHAG-MAGIC2 ... in length from the coding sequence of the gene of interest for each clone to be constructed. Only coding sequences are targeted, and each shRNA ...

In principle, cloning PCR products necessitates some fancy ... much more robust behavioral assays need to be developed for screening.” Box 1: Gene trapping for the future Gene trapping is ...

Transgenic animals continue to play an essential role in many aspects of zebrafish research, including the development of disease models. The most widely used system for zebrafish transgenesis is the ...