pH 7.4 and immersed overnight in 1.7 M sucrose and 15% PVP (polyacrylpyrolidone)/PBS. Thin sections were cut at -100 °C. The grids were floated on drops of the polyclonal anti-αB-crystallin ...

Human GBM cell lines (U87-MG, A172, and U251) were purchased from ATCC Company (Milan, Italy). Cells were suspended in Dulbecco’s modified Eagle’s medium (DMEM) (cat. no. 11965092) containing 10% ...

At 3 days, the non-attached cells/tissue were washed away using PBS, and the attached cells were cultured with DMEM containing 10% FBS and 1% P/S. The culture medium was changed every 2–3 days. The ...