13 Briefly, the samples were further diluted in 10 mM Tris-HCl/10 mM EDTA buffer to a final volume of 3 mL. Cell lysis was achieved by adding SDS (final concentration 0.6%) and 30 min of incubation at ...

Most additives are only permitted to be used in certain foods and are subject to specific quantitative limits, so it is important to note this list should be used in conjunction with the appropriate ...

Laboratory of Nanoscale Biology (LBEN), Institute of Bioengineering, School of Engineering, Swiss Federal Institute of Technology Lausanne (EPFL), 1015 Lausanne, Switzerland ...

Federal University of Pampa, Campus Uruguaiana, Uruguaiana, Rio Grande do Sul 97508000, Brazil Department of Biochemistry, Federal University of South Fronteira, Campus Chapecó, Chapecó,89815-899Santa ...

In re-ChIP experiments, the first ChIP (H2A.Z or H2A) was eluted with 10 mM Tris-EDTA and 20 mM DTT and diluted 20 times in dilution buffer (0.01% SDS, 1.1% Triton X-100, 1.2 mM EDTA, 16.7 mM Tris-HCl ...

Cells were washed with PBS, resuspended in cytoplasm extract buffer (20 mM Hepes, 10 mM KCl, 0.1 mM EDTA, 1 mM DTT ... The supernatant was removed, and the nuclei pellet was lysed in PBE150Na (50 mM ...

This experiment revealed interaction between ETT and IAA, while control experiments titrating IAA into buffer without protein and titrating buffer without IAA into the ETT fragment showed no heat ...

Loaded proteins were purified using a buffer composed of 20 mM Tris-HCl pH 7.5, 150 mM KCl, 6 mM n-decyl-ß-D-maltoside (DM), 2 mM TCEP, 2 mM DTT, 1 mM EDTA and 0.1 mg/ml of lipid mixtures described ...