After 48 h, the medium was removed and the cells were washed and lysed. Luciferase activity was measured using a luciferase assay kit, according the manufacturer’s instructions (Promega, Madison, USA) ...

PPA2 inhibits glycolysis-mediated CRC metastasis by facilitating the degradation of HIF-1α To investigate the impact of NEDD4 phosphorylation at T758 on its ubiquitination and protein degradation ...

Edited by Donald Ort, University of Illinois at Urbana Champaign, Urbana, IL; received December 10, 2024; accepted January 28, 2025 ...

Faculty of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates, South China ...

He extracted two components from beetle abdomens, naming the substrate luciferine and the enzyme responsible for the reaction luciferase. 2 Not all bioluminescent marine organism systems can be used ...

β-Actin was used as a loading control by stripping the membranes and reblotting with an anti-β-actin antibody. The luciferase reporter assay was performed in 48-well plates as described previously (14 ...

Metabolites are major biological parameters sensed by many cell types in vivo, whether they function as signaling mediators of SC and niche cross talk to regulate tissue regeneration is largely ...

We sought to minimize these challenges by developing simple biochemical assays that reproduce an aspect of exosome biogenesis and miRNA ... Furthermore, the rate of miR-223 packaging mirrored the rate ...