CD19 + B cells were isolated by human CD19 microbeads (Miltenyi Biotec, USA). The steps of cell surface staining are as follows. Initially, Fc receptor blockade was conducted on the cells for 10 ...

Ten percent of the samples were saved for immunoblotting. The other 90% of samples were separated by SDS-PAGE, visualized by colloidal coomassie blue staining, destained and subjected to mass ...