We assessed six cell lines known to harbor Nras gene mutations using our multicolor multiplex assay. Two of the cell lines, MOLT-4 and PA-1, contain mutations in codon 12, while the remaining four ...

Real-time PCR is the favored method for measuring gene ... To help researchers configure single- or multiplex assays, the Promega website will have a Plexor Primer Design tool that will recommend ...

PCR-Machines are based on Polymerase Chain Reaction (PCR) and produce copies of genes exponentially. The PCR cycling reactions are carried out in an automated thermocycler, which is capable of ...

A Chinese research team has successfully developed a triplex real-time quantitative fluorescence PCR method capable of ...

real-time PCR (qPCR), digital PCR (dPCR), reverse transcription PCR (RT-PCR), hot-start PCR, multiplex PCR, and other PCR techniques. The qPCR segment holds the largest share of the market.

Real-time PCR detects and quantifies DNA as the reaction progresses. This quantifies the specific amount of DNA present in a sample. The procedure requires specialized thermal cyclers with ...