We therefore developed a strategy to assemble the full–length HIV–1 clones by using PCR–mediated recombination (Fig. 1). The four DNA fragments (A, B, C and D) encompassing the full–length ...

sequence can be used for all fragments. The method can even be multiplexed, if the various PCR products can be size-fractionated sufficiently. Even old, degraded DNA yields enough signal to ...

PCR involves the enzymatic amplification of nucleic acid templates, and can be divided into four major steps, listed below. 1 Denaturation: Double-stranded DNA separates into single strands. Annealing ...

Some of the added genetic fragments are used for building DNA strands during amplification, while the others are used for building the DNA and adding marker labels to the strands, which are then used ...

PCR products, and proteins. OligoPrep can be used to separate double-stranded DNA fragments from 5 to 3,000 bp; it can also separate proteins from 8 to 220 kD. Usable samples are obtained immediately ...

Instruments Used in DNA Fragment Analysis The DNA sequencing instruments in the CBFG can also be used for several different kinds of DNA fragment analysis. We use: Real-time PCR (polymerase chain ...