We lysed cells in IPH buffer (1 ml; 50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 5 mM EDTA, 0.5% NP-40, 0.1 mM PMSF) at 4 °C for 30 min and removed debris by centrifugation. The cleared lysate was then ...

随后，缓慢的冷却促进了杂交反应，最终在互补的序列间形成新的氢键。 Trizma® 碱：Tris（三羟甲基氨基甲烷）的商名 Oligo：寡核苷酸或寡聚物的缩写。寡核苷酸是短单链DNA或RNA分子，必须对其进行退火（加热或解链），才可与适当的互补DNA或RNA链结合并形成双链。

Roshni Nadar Malhotra, daughter of HCL founder Shiv Nadar, became India's richest woman and the third-richest person overall after acquiring 47% of her father's stake in HCL Corporation and Vama ...

HCL Group founder Shiv Nadar has gifted 47 percent of his stake in HCL Corp and Vama Delhi, the promoter entities of the group, to his daughter Roshni Nadar Malhotra as part of a well-planned ...

Roshni Nadar Malhotra, the chairperson of HCL Technologies, has become the third richest Indian after inheriting 47% of her father Shiv Nadar's stake in HCL Corporation. Born in 1982 and raised in ...

Roshni Nadar Malhotra has cemented her place as India's third-richest individual following a major succession move within the HCL Group. She received 47% of her father Shiv Nadar's stake in HCL ...

Tris-HCl（三羟甲基氨基甲烷盐酸盐）是一种广泛使用的缓冲剂，其在中性至弱碱性条件下（pH 7.0–9.0）具有稳定的缓冲能力。20 mmol/L浓度和pH 7.8的配制参数使其适用于多种科研场景，尤其在分子生物学、生物化学和细胞学研究中具有重要价值。 · DNA/RNA稳定性与 ...

Nadar—who cofounded the software giant almost five decades ago—transferred his 47% stake in HCL Corp. and Vama Delhi to Malhotra through gift deeds. This makes Malhotra the majority ...

Mr Nadar passed the reins to his daughter, Roshni in July 2020. In a strategic succession plan, HCL Group Founder Shiv Nadar has gifted 47% of his stake in HCL Corp and Vama Sundari Investments ...

HCL Group founder Shiv Nadar transferred 47% of his stake in HCL Corp and Vama Delhi—two key promoter entities of the group—to his daughter Roshni Nadar. (Image/IE) In a major leadership ...

HCL group founder Shiv Nadar has gifted 47 per cent of his stake in HCL Corp and Vama Delhi, the promoter entities of the group, to his daughter Roshni Nadar Malhotra, in a strategic transfer as part ...

HCL Infosystems Ltd also filed a similar stock exchange. With her stakes in Vama Delhi and HCL Corp, Roshni Nadar Malhotra will become the largest shareholder of HCL Infosystems Ltd and HCL Tech.