Bedford, MA, USA) for western blotting analysis. Briefly, the membranes were blocked with 5% bovine serum albumin (BSA) blocking buffer for 1 h at room temperature, incubated with specific primary ...

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Rockford, IL). Aliquots of prepared homogenates containing 80 μg of protein were resolved by SDS-PAGE, transferred to a membrane and blotted with specific antibodies: anti-phospho-IRE1α (ab48187), ...

The protein folding capacity of the endoplasmic reticulum (ER) is tightly regulated by a network of signaling pathways, known as the unfolded protein response (UPR). UPR sensors monitor the ER folding ...

northern blotting, and western blotting and are used by research and academic institutions, and diagnostics, biotechnology, and pharmaceutical companies. Products that are based on southern ...