下面来看具体的研究结果: NePabS 的生产与纯化:研究人员将编码欧洲亚硝化单胞菌(Nitrosomonas europaea)pABA 合酶的基因 ne1434 插入 pET15b 载体,在大肠杆菌 BL21 中进行表达和纯化。金属分析显示,纯化后的蛋白质中铁的含量较低,表明其金属辅因子不稳定 ...
Insulin-like growth factor-I receptor (IGF-IR) preferentially regulates the long-term IGF activities including growth and metabolism. Kinetics of ligand-dependent IGF-IR endocytosis determines how IGF ...
The resulting HA-fused cDNAs were PCR amplified using an Xho I-HA-upstream primer and downstream T3 primer. The PCR products were digested with Xho I and introduced into the Xho I site of pET15b ...
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