A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption, to study the properties of organic or inorganic substances.

2024年4月12日 · A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to study the properties of organic or inorganic substances.

2020年5月18日 · Fluorescence microscopy is a light microscope that works on the principle of fluorescence. A substance is said to be fluorescent when it absorbs the energy of invisible shorter wavelength radiation (such as UV light) and emits longer wavelength radiation of visible light (such as green or red light).

2025年1月26日 · The fluorescence microscope is based on fluorescence. The sample’s molecules of interest are stained with fluorescent dyes (or fluorophores), and upon exposure to light of one wavelength, the fluorophore absorbs energy and emits it at a longer wavelength.

2023年8月8日 · Fluorescence microscopy allows researchers to study intricate details within biological specimens, offering insights into dynamic processes, interactions, and localization of molecules.

The modern fluorescence microscope combines the power of high performance optical components with computerized control of the instrument and digital image acquisition to achieve a level of sophistication that far exceeds that of simple observation by the human eye.

Fluorescence microscopy is a powerful tool for modern cell and molecular biologists and, in particular, neurobiologists. It provides a window into the physiology of living cells at sub-cellular levels of resolution.

Fluorescence microscopy is a major tool with which to monitor cell physiology. Although the concepts of fluorescence and its optical separation using filters remain similar, microscope design varies with the aim of increasing image contrast and spatial resolution.