Near‐infrared imaging of phytochrome‐derived autofluorescence …
2024年3月20日 · In Arabidopsis roots, DAPI stained nuclei better with water than with medium-based condition (Figure S11c). Nonetheless, DAPI encounters limitations in penetrating the …
Protocol: an improved and universal procedure for whole-mount ...
2015年10月28日 · The excellent tissue preservation is demonstrated by labeling of microtubules and actin in the elongation zone of Arabidopsis roots ... 800 (shown in green color) (panel A) …
Localization of flavonoid enzymes in Arabidopsis roots
2001年12月23日 · These patterns are also similar to what has been observed for colocalization of the vacuolar targeting proteins, AtPEP12p and AtELP, in Arabidopsis root cells (Sanderfoot et …
Does anyone knows how to stain Arabidopsis leaves nuclei with Dapi …
To visualize cell boundaries in different layers of the root sample, I am planning to counter stain my samples with propidium iodide and DAPI. I will be taking images using 3i spinning disc ...
A simple and robust protocol for immunostaining Arabidopsis pollen nuclei
2019年1月22日 · Here, we describe a simple and robust protocol to isolate Arabidopsis pollen nuclei and immunostain for histone marks. Plant growth aside, the protocol can be performed …
A protocol for combining fluorescent proteins with histological stains ...
2017年11月24日 · (a) Auramine O staining combined with CASP1::CASP1-mCherry (magenta). (b) Auramine O (green) combined with Calcofluor White (cyan) and UBQ10::H2B-mCherry …
Protocol for rapid clearing and staining of fixed Arabidopsis …
a DAPI treatment of an ovule exhibiting a two-nuclear embryo sac (stage 3-II ; ... staining root tip nuclei with TO-PRO-3 iodide resulted in poor stain penetration and diffuse intracellular signal. …
Colocalization of mCherry and GFP constructs. Confocal image of …
In 2010, one protocol for high-throughput transient transformation of Arabidopsis root epidermal cells was restored (Van Loock et al., 2010). Authors showed that the Agrobacterium infiltration ...
Whole-Mount DAPI Staining and Measurement of DNA Content …
Whole-Mount DAPI Staining and Measurement of DNA Content in Plant Cells ... in Arabidopsis by Detlef Weigel and Jane Glazebrook. Cold Spring Harbor Laboratory Press, Cold Spring …
Isolation of Nuclei Tagged in Specific Cell Types (INTACT) in
2023年8月5日 · The INTACT system at the Arabidopsis SAM. (a) Illustration of the INTACT procedure.(b, c) Microscopy images of an inflorescence of the SAM-specific INTACT line in (b) …