To determine macrophage identity we analyzed the expression of genes highly expressed in macrophages, including genes used in zebrafish as macrophage markers (e.g. csf1ra, mfap4), chemokine and pathogen recognition receptors (e.g. marco, mrc1, tlr1), and myeloid transcription factors (e.g. irf8, spi1a, cebpb), but we did not observe major ...

2024年6月11日 · Macrophages play crucial and versatile roles in regulating tissue repair and regeneration upon injury. However, due to their complex compositional heterogeneity and functional plasticity, deciphering the nature of different macrophage subpopulations and unraveling their dynamics and precise roles during the repair process have been challenging. With its distinct advantages, zebrafish (Danio ...

Macrophages In Zebrafish Tissue Regeneration. The zebrafish innate immune system has previously been described by Herbomel and colleagues . At one day post-fertilization (dpf), embryos already possess functional phagocytes capable of eliminating exogenous microorganisms.

2023年11月22日 · In zebrafish, macrophages arise in two waves of specification and colonization. Loss of colony-stimulating factor 1 receptor a (Csf1ra) ablates primitive macrophages, which arise from the anterior lateral plate mesoderm in the early embryo ~16 hours postfertilization.

2020年5月5日 · This results in fewer macrophages in the zebrafish’s tissues, and an absence of these cells in the brain. Kuil et al. went on to show that new macrophages did emerge in zebrafish that were about two to three weeks old. However, unexpectedly, these new cells were not regular macrophages. Instead, they were a new recently identified cell-type ...

2015年7月8日 · Fin wounding-induced inflammation and Escherichia coli inoculation in zebrafish larvae of 3 dpf are two well-established models triggering macrophage recruitment. Using in situ hybridization, we observed that the expression of the tumour necrosis factor alpha (tnfa), a consensus marker of M1 macrophages, was induced in cells accumulated in the caudal fin and the muscle following amputation (n ...

Because of its conserved hematopoietic program and the availability of various genetic tools and fate-mapping strategies, zebrafish serves as an excellent model system to investigate the ontogeny of hematopoietic cells, including TRMs and DCs. 23, 24, 25 Although zebrafish macrophages have been extensively studied with various reporter and ...

2017年5月19日 · The molecular mechanism regulating macrophage interaction with endothelial cells during development is unclear. Here, the authors show that in zebrafish mutation of hypoxia-inducible factor-1α ...

2022年9月20日 · Macrophages are important for tissue regeneration after injury. Here, using a single cell sequencing approach in a zebrafish injury model, the authors show that macrophages transition through a ...

Primitive macrophages have been characterized in zebrafish embryos , but are gradually replaced during later stages of definitive hematopoiesis . Multiple genes have been suggested as markers for the monocyte/macrophage lineage in zebrafish, including L-Plastin [ 20 ] and Lysozyme C [ 21 ], however subsequent reports have indicated that these ...