2019年4月25日 · Therefore, the Opn-iCreERT2 driver is best suited for the generation of mutant bile ducts, while the Ck19-CreERT driver has near absolute specificity for bile duct cells and is therefore favorable for lineage tracing experiments. Keywords: Ck19; Cre; Opn; bile duct cells; cholangiocytes; knockout; lineage tracing; mouse liver.

2024年6月17日 · Here, by employing single-cell RNA-sequencing technology, transcriptome features of Krt19 + bile duct lineage cells isolated from Krt19CreERT; Rosa26R-GFP reporter mouse livers are examined. Distinct biliary epithelial cells which include adult LSCs, as well as their downstream hepatocytes and cholangiocytes are identified.

南模生物为该研究提供了 Fah-flox, CK19-2A-CreERT2及R26-NICD-GFP基因修饰小鼠。 成体肝稳态中，肝细胞处于静息状态，增殖缓慢；当肝脏发生损伤后，肝细胞重新进入细胞周期，参与损伤修复，维持肝脏功能。

2019年1月4日 · Using a tamoxifen-inducible Cre recombinase (CreERT2) expression system, we examined the effect of spontaneous Spint2 deletion on mice 6 weeks after birth. We found that Hai-2 is an essential...

2016年4月1日 · Here, we have established a novel mouse model of ICC by liver-specific Kras activation and Pten deletion. An activating mutation of Kras in combination with deletion of Pten was introduced in...

Various epithelial tissues, including the intestines and liver, were stained for Epcam. Intrahepatic bile ducts are indicated by arrowheads, and the inset shows the high-magnification image.

2022年3月4日 · We propose that biliary tuft cells function as bile acid–sensitive negative regulators of inflammation in biliary tissues and serve to limit inflammation under homeostatic conditions. Bile acids (BAs) are required for uptake of fats and fat-soluble vitamins and serve as signaling molecules (1).

2014年5月1日 · Using a knockin (KI) mouse strain where the inducible recombinase CreERT2 is inserted into the Sox9 locus, cholangiocytes, but not hepatocytes, were lineage-labeled in the normal adult liver. The label gradually spread out to hepatocytes from the periportal toward pericentral regions and eventually occupied nearly the whole parenchyma after 1 year.

2023年6月13日 · The expression of genes involved in the mTORC1 pathway was increased in biliary spheroids from Hnf1b-CreERT2; Kras LSL-G12D; Rosa26 LSL-NotchIC mice and inhibition of the mTORC1 pathway suppressed spheroid growth. Additionally, simultaneous activation of the PI3K-AKT and Notch pathways in EHBD and GB induced biliary cancer development in mice.

Scale bar, 100 μm. C Schematic of the heterozygous multicolor Alb-CreERT2 Rosa26rbw mice, that allow us to trace the fate of polyploid or diploid hepatocytes in vivo.