For light microscopy, there are three general approaches to visualizing mitochondrial structures: 1) labeling mitochondria in live cells with fluorescent dyes, 2) immunostaining fixed cells with …

2012年2月29日 · Transmission electron microscopy (left) shows the complex internal membrane structure of mitochondria, and electron tomography (right) gives a three-dimensional view. …

2023年1月18日 · Mitochondria are extremely pleomorphic organelles. Automatically annotating each one accurately and precisely in any 2D or volume electron microscopy (EM) image is an …

2020年4月11日 · To do this we used electron microscopy to visualise a reaction that assesses the activity of cytochrome c oxidase in the mitochondrial respiratory chain. The reaction deposits a …

2025年1月22日 · Mitochondria are rod-shaped structures ranging from 2 to 8 micrometers in length surrounded by two membranes. Mitochondria are located throughout the cytoplasm. ...

2020年7月24日 · Conventional fluorescence microscopy can only provide ~300 nm resolution, which is insufficient to visualize mitochondrial cristae. Here, we developed an enhanced …

2014年6月1日 · To evaluate what can be expected when imaging mitochondria with conventional diffraction-limited microscopy or diffraction-unlimited nanoscopy, we simulated three simplified …

Mitochondria have a distinctive appearance when viewed by electron microscopy. They often appear as rounded or sausage-shaped structures (Figure 1a, b and Figure 22a, b), measuring …

In this review, we provide an overview on recent studies using super-resolution microscopy to investigate mitochondria, discuss the strengths and opportunities of the various methods to …

2024年9月9日 · In this issue, Ren et al. introduce HBmito Crimson, a fluorogenic and photostable mitochondrial probe for STED microscopy and investigate how mitochondrial dynamics …