2021年7月26日 · Multiplex PCR is a technique whereby PCR is used to amplify several different DNA sequences simultaneously. It is a type of target enrichment approach. It was first described in 1988 as a method to detect deletion mutations in the dystrophin gene – …

2019年3月1日 · The multiplex PCR often abbreviated as mPCR is an excellent variant of the conventional PCR in which using different sets of primers, different DNA templates or various regions of a gene can be amplified, in a single reaction.

Multiplex polymerase chain reaction (Multiplex PCR) refers to the use of polymerase chain reaction to amplify several different DNA sequences simultaneously (as if performing many separate PCR reactions all together in one reaction).

Multiplex PCR is a variant of PCR methods in which more than one target sequence are amplified using multiple sets of primers within a single PCR mixture. This enables amplification of several gene segments at the same time, instead of specific test runs for each.

Anchored multiplex PCR (AMP-PCR) is a highly efficient molecular biology technology used in genomics, transcriptomics, and pathogen detection that enables simultaneous detection of multiple target sequences in a single experiment, saving time and resources.

Explore Bio-Rad qPCR and RT-qPCR reagents for multiplex PCR and discover the key to optimizing your multiplex PCR assays. Multiplex PCR is the simultaneous detection of multiple targets in a single reaction well, with a different pair of primers for each target.

The multiplex polymerase chain reaction (Multiplex PCR) refers to the use of PCR to amplify several different DNA sequences simultaneously using multiple primers in one tube and one amplification program for all amplicons.

Multiplex PCR is becoming a rapid and convenient screening assay in both the clinical and the research laboratory. The development of an efficient multiplex PCR usually requires strategic planning and multiple attempts to optimize reaction conditions.

Multiplex PCR involves the simultaneous amplification of two or more primer sets in a single reaction. Because of the increased number of primers in the reaction, proper primer design is critical to the success of your multiplex reaction.

Learn the advantages of multiplexing, duplexing and how to perform multiplex qPCR to measure the expression of three or four genes simultaneously. Read about considerations when multiplexing and primer limitation.