2009年4月1日 · Purified PBS complexes usually dissociate, unless high concentrations of phosphate are present. However, high phosphate concentrations interfere with the standard negative staining of samples for EM and often lead to noisy backgrounds. A fixation procedure is therefore required in order to remove phosphate before staining.

We found that PBSs from Anabaena 7120 yielded the best results with negative-stain EM. We also performed a cryo-EM study, but obtained poorer results due to the low contrast (possibly because of the high salt concentration in the buffer) and the preferred orientation (only the end-on view shown in vitreous ice).

2021年9月1日 · In this study, we examined the overall structure of the PBS complex isolated from T. vulcanus by single-particle analysis using negative-staining EM. Our results showed three trimers in the A1 and A2 cylinder, which is clearly different …

2005年7月4日 · Phycobilisomes are protein complexes that harvest light and transfer energy to the photo system. Here, the three dimensional structure of intact phycobilisomes from Nostoc flagelliforme is studied by a combination of negative stain …

2021年9月17日 · Both PBS contain phycocyanin (PC), allophycocyanin (APC), and linker proteins but lack phycoerythrin in red algal PBS. Negative-staining electron microscopy (nsEM) analysis revealed a large...

2023年3月15日 · The negative-staining EM structures of the PBS–PSII supercomplex and PBS–CpcL–PSI supercomplex purified from Anabaena sp. PCC 7120 showed the association of PBS with PSII 21 and PSI tetramer ...

2009年2月1日 · In this work we have studied, using single particle electron microscopy, the phycobilisome structure in mutants lacking either two or all three of the phycocyanin hexamers. The images presented...

2021年11月10日 · The fractions of intact phycobilisomes are confirmed by 77K fluorescent emission spectrum and SDS-PAGE analysis. The resulting phycobilisome fractions are suitable for negative staining of TEM and mass spectrometry analysis.

2005年6月2日 · Phycobilisomes are protein complexes that harvest light and transfer energy to the photo system. Here, the three dimensional structure of intact phycobilisomes from Nostoc flagelliforme is studied by a combination of negative stain …

2021年11月10日 · The fractions of intact phycobilisomes are confirmed by 77K fluorescent emission spectrum and SDS-PAGE analysis. The resulting phycobilisome fractions are suitable for negative staining of TEM and mass spectrometry analysis.