In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time adding restriction sites to the ends of that piece of DNA so that it can be easily …

TOPO cloning allows PCR products to be rapidly joined to linearized plasmid vectors by the enzyme topoisomerase I, without the requirement for DNA ligase. There are three types of …

2016年3月29日 · In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time adding restriction sites to the ends of that piece of DNA so that it …

2023年6月20日 · Often, the first step in a molecular biology experiment is to clone (i.e. copy) a gene into a plasmid, then transform this recombinant plasmid back into bacteria so that …

Digest plasmid with the appropriate restriction enzymes to produce a DNA fragment that can be cloned directly into a vector. Unidirectional cloning is achieved with restriction enzymes that …

Molecular cloning or the creation of recombinant DNA is an essential process used in scientific research and discovery. With molecular cloning scientists can amplify and manipulate genes …

A wide variety of protocols from Addgene that can be used for basic molecular biology, plasmid cloning, and titering and testing your viral preparations.

Explore plasmid DNA's role in molecular cloning, gene therapy and bioengineering. Learn about plasmid design, construction & uses in protein production.

2021年3月15日 · We developed and standardized a plasmid cloning protocol based on a universal MCS (Multiple Cloning Site) design and bacterial in vivo assembly. With this method, …

When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible …