CXCL16 is a single-pass type I transmembrane glycoprotein that can be enzymatically cleaved to generate a soluble form. CXCL16 is expressed by some macrophages and dendritic cells. Transmembrane CXCL16 can function as a scavenger receptor for …

Antibodies that detect CXCL16 can be used in several scientific applications, including Western Blot, ELISA, Immunohistochemistry, Flow Cytometry and Immunocytochemistry. These antibodies target CXCL16 in Human, Mouse, Rat and Non-human primate samples.

CXCL16 plays a dual role in chemotaxis (sCXCL16-CXCR6 interaction) and adhesion (mCXCL16-CXCR6, and sCXCL16-mCXCL16 interaction) with both soluble and transmembrane forms. CXCL16 expression is increased by pro-inflammatory cytokines (e.g., TNF-α, IFN-γ, IL-1β, IL-6) and transcription factors (e.g., HIF-1 α).

Flow cytometric analysis of CXCL16 expression on human peripheral blood monocytes. Human whole blood was stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human CXCL16 …

Cell surface CXCL16 can be shed in a soluble form after proteolytic cleavage to exert its chemotactic function. CXCL16 is differentially expressed by keratinocytes, monocytes, dendritic cells, endothelial cells, and some activated T cells.

2024年12月24日 · To validate the augmented levels of M-MDSCs in PJI by scRNA analysis, we performed flow cytometry on knee synovial cells from PJI, OA, and AF patients. The results revealed the ratio of myeloid cells were similar among the three groups, however, an increasing number of M-MDSCs were observed in the PJI group ( p < 0.01) (Figure 2j–l ).

2009年5月4日 · CXCL16 expression was detected using real-time reverse transcriptase–polymerase chain reaction (RT-PCR), Western blotting, enzyme-linked immunosorbent assay (ELISA), and flow cytometry. Chemotaxis assays were used to test functionality of the secreted protein.

2024年1月17日 · CXCL16 is expressed on the surface of immunosuppressive myeloid cells. CXCL16 has transmembrane and soluble forms that can control the migration and function of CXCR6 + cells. We used flow cytometry to validate how different immune cell populations express CXCL16 in CT2A tumor-bearing mice (Figure 2). Two weeks after tumor injection, we ...

2024年5月24日 · In the context of pulmonary fibrosis or TGF-β1 stimulation in vitro, macrophages exhibited an M2-polarized phenotype and secreted more CXCL16 than those of the control group. Moreover, flow cytometry revealed increased expression levels of CD69 and CXCR6 in pulmonary CD4 T cells during fibrosis progression.

2024年5月15日 · Using both RT-qPCR (Figure S3F) and flow cytometry (Figures 2D, S3G, and S3H), we found that islet macrophages in both strains exhibited the highest levels of CXCL16 total expression. These data suggest a strong association of CXCL16 expression with the islet microenvironment.