Here, we successfully implemented a robust, simple, and affordable approach to sequence monoclonal antibody variable regions from RNA with a turn-around time of five days at a cost of $70 –$120 per antibody. Results Monoclonal antibody sequencing strategy

2019年6月24日 · Here, we describe a simplified workflow for amplification of IgG antibody variable regions from hybridoma RNA by a specialized RT-PCR followed by Sanger sequencing. We perform three separate reactions for each hybridoma: one each for kappa, lambda, and heavy chain transcripts.

In the case of the B-cell receptor the C-terminus is a hydrophobic membrane-anchoring sequence, and in the case of antibody it is a hydrophilic sequence that allows secretion. Since they are soluble, and secreted in large quantities, antibodies are …

Antibody sequence information is crucial to understanding the structural basis for antigen binding and enables the use of antibodies as therapeutics and research tools. Here, we demonstrate a method for direct de novo sequencing of monoclonal IgG from the purified antibody products.

T-cell receptor (TCR) interaction with major histocompatibility complex–antigen complexes leads to antitumour responses. TCR sequencing analysis allows characterisation of T cells that recognise tumour neoantigens. T-cell clonal revival and clonal ...

We demonstrate the power of AbLang by using it to restore missing residues in antibody sequence data, a key issue with B-cell receptor repertoire sequencing, e.g. over 40% of OAS sequences are missing the first 15 amino acids.

Gene expression profiling by RNA-Seq provides an unprecedented high-resolution view of the global transcriptional landscape. As the sequencing technologies and protocol methodologies continually evolve, new informatics challenges and applications develop.

2024年3月16日 · To accomplish this, the authors performed deep sequencing analysis of Ab repertoires from both naïve and immunized mice. Via this process, they were able to determine the critical Ab sequence features associated with target antigen affinity interactions.

A large number of applications are supported, including targeted and de novo DNA and RNA sequencing, transcriptome sequencing, microbial sequencing, copy number variation detection, small RNA and miRNA sequencing and CHIP-seq …

Sequencing analysis. The analysis of antibody sequences (Figures 2D–2F) provides the following information: Clonal families: Clones are defined by antibodies sharing the same heavy and light germline and by calculating the hamming distance for …