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活性氧检测 - Bio-protocol
实验原理：当过氧化物酶存在时，二氨基联苯胺 (3,3’-diaminobenzidine, DAB) 被双 氧水氧化生成不溶于水的暗棕色颗粒。 实验目的：定性、原位检测水稻各组织中的双氧水。
Detection of Hydrogen Peroxide by DAB Staining in - PMC
DAB is oxidized by hydrogen peroxide in the presence of some haem-containing proteins, such as peroxidases, to generate a dark brown precipitate. This precipitate is exploited as a stain to detect the presence and distribution of hydrogen peroxide in plant cells.
Detection of Hydrogen Peroxide by DAB Staining in Arabidopsis Leaves
DAB is oxidized by hydrogen peroxide in the presence of some haem-containing proteins, such as peroxidases, to generate a dark brown precipitate. This precipitate is exploited as a stain to detect the presence and distribution of hydrogen peroxide in plant cells.
Fluorescent in vivo imaging of reactive oxygen species and redox ...
2018年7月1日 · Another popular colorimetric in situ ROS indicator is 3,3´-diaminobenzidine (DAB), which reacts with H 2 O 2 in a peroxidase-catalysed reaction rendering a brown precipitate that can be microscopically imaged at tissue and cellular levels [32].
Determination of ROS Generated by - Springer
2024年4月9日 · In this protocol, we describe the use of NBT and DAP staining to detect ROS generation under different stresses such as nitrogen starvation, wounding, or UV-C. Additionally, we describe the use of NBT staining for detecting enzymatic generation of ROS in native and native SDS PAGE gels.
Highlighting reactive oxygen species as multitaskers in root ...
2021年1月22日 · DAB and NBT are not specific or direct measurements of both ROS, and color formation does not always reflect the levels of the desired ROS. NBT staining can reflect the presence of ascorbate or the activity of dehydrogenases, while DAB brownish color accumulates in the presence of higher peroxidase activity.
Fig. 5. ROS detection in root cells. AeF: Diaminobenzidine...
Diaminobenzidine tetrahydrochloride (DAB) staining assays (Litwin, 1979) also revealed that the root cells from pABI3:BLX/blx/À plants exhibited significantly stronger DAB staining than...
DAB staining to detect the presence of ROS in whole leaves and …
After uptake of DAB, the stain reacts with H2O2 in the plant cells to form a reddish-brown polymer in the presence of peroxidase [30,31]. ...
Determination of ROS Generated by Arabidopsis Xanthine …
In this protocol, we describe the use of NBT and DAP staining to detect ROS generation under different stresses such as nitrogen starvation, wounding, or UV-C. Additionally, we describe the use of NBT staining for detecting enzymatic generation of ROS in …
ROS Detection and Quantification in Plant–Nematode Interactions
Several methods to analyze the cellular redox state exist, among which ROS detection and quantification and the evaluation of ROS scavenging enzyme activity (peroxidase activity, catalase activity, etc.). Here, we describe DAB staining, which is used to detect and localize ROS in planta upon an external trigger.