DAPI (4′,6-diamidino-2-phenylindole) is a blue-fluorescent DNA stain that exhibits ~20-fold enhancement of fluorescence upon binding to AT regions of dsDNA. It is excited by the violet (405 nm) laser line and is commonly used as a nuclear counterstain in fluorescence microscopy, flow cytometry, and chromosome staining.

Performing nuclear staining with DAPI or Hoechst? Check out Biotium's detailed Hoechst & DAPI staining protocols for staining live cells or fixed cells & tissues.

DAPI (pronounced 'DAPPY', /ˈdæpiː/), or 4′,6-diamidino-2-phenylindole, is a fluorescent stain that binds strongly to adenine–thymine-rich regions in DNA. It is used extensively in fluorescence microscopy.

2011年1月1日 · A number of fluorescent stains are available that label DNA and allow easy visualization of the nucleus in interphase cells and chromosomes in mitotic cells, including Hoechst, 4',6-diamidino-2-phenylindole (DAPI), ethidium bromide, propidium iodide, and …

A simple-to-use fluorescent stain, 4',6-diamidino-2-phenylindole (DAPI), visualizes nuclear DNA in both living and fixed cells. DAPI staining was used to determine the number of nuclei and to assess gross cell morphology.

DAPI is a popular nuclear counterstain for use in multicolor fluorescent techniques. Its blue fluorescence stands out in vivid contrast to green, yellow, or red fluorescent probes of other structures.

The blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; it appears to asso- ciate with AT clusters in the minor groove. 1 Binding of DAPI to dsDNA produces a ~20-fold fluorescence enhancement, apparently due to the displacement of water molecules from

A number of fluorescent stains are available that label DNA and allow easy visualization of the nucleus in interphase cells and chromosomes in mitotic cells, including Hoechst, 4′,6-diamidino-2-phenylindole (DAPI), ethidium bromide, propidium iodide, and acridine orange.

2017年3月6日 · DAPI fluorescence staining was used to calculate the amount of DNA in a nucleus and, subsequently, infer its cell cycle status, through a k-means clustering approach. The area of each...

2018年10月1日 · DAPI specifically stains double stranded DNA with no non-specific labeling in the cytoplasm. For DAPI to enter cells and bind to DNA, the cells need to be permeabilized.