Differential interference contrast (DIC) microscopy, also known as Nomarski interference contrast (NIC) or Nomarski microscopy, is an optical microscopy technique used to enhance the contrast in unstained, transparent samples.

Unlike phase contrast, differential interference contrast converts gradients in specimen optical path length into amplitude differences that can be visualized as improved contrast in the resulting image.

2025年1月23日 · DIC is differential interference contrast, an optical contrast technique for microscopy which allows unstained structures in the cells of biological specimens to be observed with adequate contrast and resolution.

2024年11月21日 · How differential interference contrast (DIC) is a good alternative to brightfield illumination when using microscopy to image transparent, unstained biological specimens is explained in this article.

Differential Interference Contrast (DIC) is a microscopy technique that introduces contrast to images of specimens which have little or no contrast when viewed using brightfield microscopy. This guide explains how to set up DIC on an upright microscope.

DIC is especially useful in fluorescence and confocal microscopy to indicate the morphological aspect of fluorescent regions. DIC reveals some ultrastructural features of cells such as microtubules and cytoplasmic granules.

Through a mechanism different from phase contrast, differential interference contrast converts specimen optical path gradients into amplitude differences that can be visualized as improved contrast in the resulting image.

An excellent mechanism for rendering contrast in transparent specimens, differential interference contrast (DIC) microscopy is a beam-shearing interference system in which the reference beam is sheared by a minuscule amount, generally somewhat less than the diameter of an Airy disk.

Also known as Nomarski microscopy or imaging, differential interference contrast microscopy takes advantage of differences in the light refraction by different parts of living cells and transparent specimens and allows them to become visible during microscopic evaluation.

2016年2月25日 · An excellent mechanism for rendering contrast in transparent specimens, differential interference contrast (DIC) microscopy is a beam-shearing interference system in which the reference beam is sheared by a minuscule amount, generally somewhat less than the diameter of an Airy disk.