In this mini-review, we address exosome manufacturing challenges, including cell line development, upstream cell culture, and downstream purification processes in pre-clinical and clinical activities.

Production method of exosomes for improving the productivity base on a serum-free medium, to which platelet lysate is added (0.01–20%). The productivity of the number of exosome particles per ml of culture medium is approximately two or more times higher that without treatment.

2022年7月1日 · The schematic illustration of drug-loaded exosomes process steps. When using exosomes as drug delivery vehicles, it's important to have a high-throughput exosomes source of cells as well as a reproducible and scalable production and isolation protocol.

Two main strategies are used to increase exosome production, one is based on genetic manipulation of the exosome biogenesis and release pathway, and the other is by pretreating parent cells, changing the culture method or adding different components to the medium.

2022年5月24日 · Exosomes are nanovesicles with different contents that play a role in various biological and pathological processes. It offers significant advantages over other delivery systems such as liposomes and polymeric nanoparticles.

2022年3月25日 · Exosomes are extracellular vesicles (EVs) produced in endosomes of eukaryotic cells and have attracted attention in life sciences research and biotechnology because they participate in intercellular communication in various normal and pathological functions.

2022年5月31日 · In this mini-review, we will discuss the manufacturing challenges of therapeutic exosomes, including cell line development, upstream cell culture, and downstream purification process.

2022年7月1日 · Exosomes are nanovesicles, 20–100 nm in size, and they can be released from various cells such as fibroblasts, immune system cells (T-cells, B-cells, dendritic cells), adipocytes, and tumor cells and isolated from all normal and diseased body fluids such as blood, urine, breast milk, amniotic fluids, and bronchial alveolar lavage.

exosome sub-populations; ii) scalable cell culture methods for upstream production of 121 exosomes; and iii) scalable downstream processing for isolation and purification of exosomes.

Discover our bioreactor control systems and centrifuges for exosome process development, scale-up of exosome production, and exosome purification.