The BD ® Spectrum Viewer is an interactive tool that supports optimized fluorochrome selection, choosing filters and assessing potential spillover when designing flow cytometry panels.

With spectral flow cytometry, the emission spectrum of every fluorescence molecule is captured by a set of detectors across a defined wavelength range. Every molecule’s fluorescent spectrum can be recognized, recorded as a spectral signature, and used as reference in …

The new Full Spectrum Viewer features: More fluorescent tags to select from; Fluorescent tag signatures; Complexity™ Index; Similarity™ Index; Stain Index Reduction; Transfer fluorescent tags to experiment templates

What is Spectral Flow Cytometry? Spectral flow cytometry is an advanced analytical technique that enhances traditional flow cytometry by capturing the complete emission spectra of fluorochromes.

Spectral flow cytometry is an exciting technology that allows for high-dimensional analyses of cells with dozens of fluorophores in single-tube assays. Spectral flow cytometry has gained substantial traction in research settings and is now available to clinical laboratories in China and Europe.

Watch the spectral flow cytometry videos below to explore various topics, such as the fundamentals of spectral and conventional flow cytometry, how you can use more fluorochromes per laser line using spectral flow cytometry, how to use spectral flow cytometry for increased panel size, and more.

Here we provide spectral flow cytometry users a guide to selection of available fluorophores, assays and reagents that fit the needs of multiplexed spectral flow cytometry approaches in cell biology, immunology, cancer biology, microbiology, and plant biology.

In this Unit, we review the general considerations for flow-based spectral measurements, discuss the options for hardware, considerations for software, and current and future applications of spectral flow cytometry.

2024年1月24日 · The main challenge with multicolor spectral flow cytometry is spectral unmixing, i.e., the correct, quantitative identification of the fluorochromes that contribute to the overall spectrum of emitted light.

In this article we discss the 4 spectral viewers you should be using to get the best flow cytometry data and publish hihg quality results.

Full Spectrum Cytometry offers a new mode to measure fluorescence and offers lots of opportunities to expand panels and improve detection, especially in highly autofluorescent cells.

Spectral flow cytometry is a rapidly growing area of flow cytometry and, in this blog, we look at the basic principles and the advantages over conventional flow cytometry.

2024年3月13日 · Discover the transformative potential of spectral flow cytometry in biomedical research with our comprehensive guide. Learn about its key features, applications, and future perspectives in immunology, oncology, stem cell biology, and infectious diseases.

Contemporary full spectrum or "spectral" flow cytometry is a recently developed technology that allows for high-dimensional flow cytometric analyses of cells and particles in suspension.

Spectral flow cytometry enables high-dimensional single-cell evaluation of large cohorts in a high-throughput manner. Here, we designed, optimized, and implemented new methods for deep immunophenotyping of human peripheral blood and bone marrow by spectral flow cytometry.

Spectral Flow Cytometry is a technique based on conventional Flow Cytometry where a spectrograph and multichannel detector (usually CCD) is substituted for the traditional mirrors, optical filters and photomultiplier tubes (PMT) in conventional systems.

The BD ® Spectrum Viewer is an interactive tool that supports optimized fluorochrome selection, choosing filters and assessing potential spillover when designing flow cytometry panels.

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The BD Spectrum Viewer is a tool that depicts the excitation and emission curves of fluorochromes common to flow cytometry. This tool can be used to determine appropriate filters to detect a fluorochrome as well as fluorochrome compatibility and fluorescent spillover.

3 天之前 · Hundreds of fluorochromes are available to users conducting flow cytometry assays, each with unique properties that influence panel resolution and data quality. Selecting the right combination can be overwhelming, leading many researchers to rely on familiar choices that may introduce spillover and reduce clarity.

Full spectrum flow cytometry (FSFC) measures the entire fluorochrome emission spectrum, across multiple lasers and using many more detectors than a conventional flow cytometer, allowing a specific spectral fingerprint to be defined for each fluorochrome.

1 天前 · NucSpot® Nuclear Stains can also be used for selective staining of dead cells in unfixed cell cultures for analysis by flow cytometry or fluorescence imaging. Several stains can be continuously incubated with cells for multi-day imaging, further expanding researchers’ toolkits for nuclear fluorescence imaging.

The BD ® Spectrum Viewer is an interactive tool that supports optimized fluorochrome selection, choosing filters and assessing potential spillover when designing flow cytometry panels.