A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption, to study the properties of organic or inorganic substances.

2024年4月12日 · A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to study the properties of organic or inorganic substances.

2020年5月18日 · Fluorescence microscopy is a light microscope that works on the principle of fluorescence. A substance is said to be fluorescent when it absorbs the energy of invisible shorter wavelength radiation (such as UV light) and emits longer wavelength radiation of visible light (such as green or red light).

The modern fluorescence microscope combines the power of high performance optical components with computerized control of the instrument and digital image acquisition to achieve a level of sophistication that far exceeds that of simple observation by the human eye.

2025年1月26日 · Fluorescence microscopy has applications across the board. For instance, fluorescence microscopy is used in the analysis of diagnostic antibodies and antigen expression in tissue sections in biomedical research, as well as localization studies for proteins and nucleic acids in certain parts of the cell. Less extensively, microbiology, neuroscience, and …

Fluorescence microscopy is a powerful tool for modern cell and molecular biologists and, in particular, neurobiologists. It provides a window into the physiology of living cells at sub-cellular levels of resolution.

2005年11月18日 · This review attempts to provide a framework for understanding excitation of and emission by fluorophores, the way fluorescence microscopes work, and some of the ways fluorescence can be...

2024年4月2日 · A fluorescence microscope is a specialized optical instrument designed to study the properties of organic or inorganic substances using the principles of fluorescence and phosphorescence. These phenomena are distinct from reflection and absorption, which are commonly employed in traditional microscopy.

2020年12月16日 · A fluorescence microscope works by combining the magnifying properties of the light microscope with fluorescence emitting properties of compounds. Fluorescence microscopy uses a high-intensity light source that excites a fluorescent molecule called a fluorophore in the sample observed.

Learn the basic concepts of fluorescence, a member of the ubiquitous luminescence family of processes in which susceptible molecules emit light from electronically excited states created by either a physical, mechanical, or chemical mechanism.