Limulus amebocyte lysate (LAL) is an aqueous extract of motile blood cells from the Atlantic horseshoe crab Limulus polyphemus. LAL reacts with bacterial endotoxins such as lipopolysaccharides (LPS), which are components of the bacterial capsule, the outermost membrane of cell envelope of gram-negative bacteria.

2019年1月8日 · Limulus amebocyte lysate (LAL) is an aqueous extract of blood cells (amoebocytes) from the horseshoe crab, Limulus polyphemus. LAL reagent reacts with bacterial endotoxin and lipopolysaccharide (LPS), which is a membrane constituent of …

Currently the in vitro limulus amebocyte lysate (LAL) test is the assay of choice for the determination of endotoxin contamination. The LAL assay exists in three formats, namely chromogenic, turbidity, or gel-clot assessments.

鱟 hòu 試劑 （全稱 鱟阿米巴樣細胞溶解物，英語： Limulus amebocyte lysate，縮寫 LAL）是一種來自 美洲鱟 血液 中 阿米巴樣細胞 的 細菌 內毒素 脂多糖 （LPS）檢測試劑，廣泛應用於 革蘭氏陰性菌 內毒素的檢測和定量。 [1] 在亞洲，美洲鱟試劑經常被來自 南方鱟 或 中華鱟 血液的 亞洲鱟試劑 （英語： Tachypleus amebocyte lysate，縮寫 TAL）替代。 鱟試劑遇到 LPS 時會凝固成膠，為鱟的一種免疫反應。 由於制作試劑需要抽血，取血時可能會導致鱟死亡，現在有呼吁 …

The limulus amebocyte lysate (LAL) has been widely used for ~30 years for the detection of endotoxin in the quality assurance of injectable drugs and medical devices. The LAL constitutes a cascade of serine proteases which are triggered by trace levels of endotoxin, culminating in a gel clot at the end of the reaction.

2019年1月13日 · Limulus amebocyte lysate (LAL) is an aqueous extract of blood cells (amoebo-cytes) from the horseshoe crab, Limulus polyphemus. LAL reagent reacts with bacterial endotoxin and...

2009年1月1日 · This year celebrates the 30th anniversary of the licensing of Limulus amebocyte lysate (LAL) by the US Food and Drug Administration (FDA) as a test for the presence of endotoxin in biologicals, pharmaceutical drugs, and medical devices. LAL is currently recognized by several major pharmacopoeias and is used worldwide.

2021年2月18日 · In this study, we investigated which factors influence the recovery of endotoxins using limulus ameobocyte lysate (LAL)-based assays. The individual factors that were examined in more detail...

2013年6月1日 · The Limulus amebocyte lysate (LAL) test is the most widely used method for bacterial endotoxin tests. It is a simple and highly sensitive biochemical assay in which endotoxin binding to factor C starts the coagulation cascade [5].

2016年9月1日 · The limulus amebocyte lysate (LAL) assay originally developed by Levin and Bang [9], [10] is now used almost exclusively in place of the RPT for testing of endotoxins in biotechnological products upon the demonstration of the equivalency. The LAL assay as described in USP <85> has proven reliable, sensitive, and specific for general endotoxin ...