Mouse Model of Middle Cerebral Artery Occlusion - PMC
The intraluminal monofilament model of middle cerebral artery occlusion (MCAO) involves the insertion of a surgical filament into the external carotid artery and threading it forward into the internal carotid artery (ICA) until the tip occludes the origin of the MCA, resulting in a cessation of blood flow and subsequent brain infarction in the ...
Hypothalamic, thalamic and hippocampal lesions in the mouse MCAO model …
2015年10月30日 · Intraluminal monofilament occlusion of the middle cerebral artery (MCAO) in mice is the most used rodent model to study the pathophysiology of stroke. However, this model often shows brain damage in regions not supplied by the MCA such as the hypothalamus, hippocampus and thalamus.
A Modified Transcranial Middle Cerebral Artery Occlusion Model …
In experimental stroke research, middle cerebral artery occlusion (MCAO) with an intraluminal filament is widely used to model ischemic stroke in mice. The filament MCAO model typically exhibits a massive cerebral infarction in C57Bl/6 mice that sometimes includes brain tissue in the territory supplied by the posterior cerebral artery, which is ...
Modified middle cerebral artery occlusion model provides …
2021年7月1日 · Middle cerebral artery occlusion (MCAO) with 1 -h ischemia followed by reperfusion is a widely used stroke model in rodents that has significant limitations such as high mortality and severe neurological deficit hampering comprehensive neurobehavioral evaluation.
Mouse model of middle cerebral artery occlusion - PubMed
2011年2月13日 · To evaluate the extent of cerebral infarction, we stain brain slices with 2,3,5-triphenyltetrazolium chloride (TTC) to identify ischemic brain tissue. In this video, we demonstrate the MCAO method and the determination of infarct size by TTC staining.
Optimizing intraluminal monofilament model of ischemic stroke …
2022年12月9日 · Intraluminal monofilament model of middle cerebral artery occlusion (MCAO) is widely adopted for ischemic stroke; and Sprague–Dawley (SD) rats are commonly used rodents for preclinical research.
Three-dimensional visualization of cerebral blood vessels and …
2022年9月23日 · Because the middle cerebral artery is a common site of ischemic stroke, middle cerebral artery occlusion (MCAO) model is primarily used as a standard animal model for focal brain ischemia...
Intraluminal Middle Cerebral Artery Occlusion (MCAO) Model …
2011年5月8日 · To model focal cerebral ischemia, an intraluminal suture is advanced via the internal carotid artery to occlude the base of the MCA. Retracting the suture after a specified period of time mimics spontaneous reperfusion, but the suture can also be permanently retained.
Intraluminal Middle Cerebral Artery Occlusion Model in Mice
2019年7月24日 · Intraluminal middle cerebral artery occlusion (MCAO) model in rodents has been widely used for cerebral ischemia studies. The procedure is relatively simple and non-invasive compared to the other focal cerebral ischemia models, …
Optimisation of a Mouse Model of Cerebral Ischemia‐Reperfusion …
2022年7月6日 · Middle cerebral artery occlusion (MCAO) induced brain ischemia-reperfusion model in Mice is essential for understanding the pathology of stroke and investigating potential treatments, in which a variety of methods may be employed to block the middle cerebral artery (MCA), the most common being through the insertion of a monofilament; however, in...