NF-κB p65 Antibody - Cell Signaling Technology
NF-κB p65 Antibody detects endogenous levels of total NF-κB p65. This antibody may also cross-react with the p50 subunit. The antigen sequence used to produce this antibody shares 100% …
Monitoring the Levels of Cellular NF-κB Activation States
As shown in Figure 3B,D, experiments addressing the protein expression and post-translational modifications of NF-κB components are typically performed by Western blotting and thus …
NF-κB p65 (RELA)靶点介绍及实验小贴士| Abcam中文官网
nf-κb受翻译后修饰和亚细胞区室化的各种机制以及与其他辅因子或共抑制因子的相互作用的控制。 未活化的nf-κb存在于细胞质中，与其抑制剂iκb结合；在传统激活途径中，iκb被ikk磷酸化后降 …
Quantifying Nuclear p65 as a Parameter for NF-κB Activation ...
With increasing fractions of TNFα treated cells, the western blot analysis qualitatively and quantitatively demonstrates the expected increase in the nuclear NF-κB/p65 (Figure 5A+B). …
Quantifying nuclear p65 as a parameter for NF-κB activation ...
It is demonstrated that ImageStream assessment of receptor-mediated (TNFα) and drug (Daunorubicin, DNR)-induced NF-κB translocation in leukemic cell lines correlates well with …
Western blot analysis of NF-kB and pNF-kB. Protein expression of NF-kB …
Protein expression of NF-kB and pNF-kB in BJ+ SK-MEL-28 co-culture treated with trametinib (25 nM) and celecoxib (50 nM). WB bands were analyzed by densitometry and results were …
Measurement of NF-κB activation in TLR-activated macrophages
Two techniques have been developed to test for NF-κB translocation: 1) Cell fractionation, in which cytoplasmic and a nuclear fractions are isolated and NF-κB protein is quantified by …
NF-κB Pathway Antibody Sampler Kit - Cell Signaling Technology
Simple Western™ analysis of lysates (1.0 mg/mL) from HeLa cells treated with hTNF-α (20 ng/mL, 5 minutes) using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033. The virtual …
Phospho-NF-κB p65 (Ser536) Antibody - Cell Signaling …
Polyclonal Antibody for studying NFkB-p65 (Ser536) phosphate. Validated for WB. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-NF-κB p65 (Ser536) …
Western blot analysis of the expression and activation of NF-kB ...
We found that obese males and females showed higher NF-kβ p-65, TNF-α and MCP-1 in renal tissue, and obese females ovariectomized had higher IL-1β and TNF-α compared with not …