A typical aptamer is a synthetically generated ligand exploiting the combinatorial diversity of DNA, RNA, XNA, or peptide to achieve strong, specific binding for a particular target molecule or family of target molecules.

RNA aptamers, small oligonucleotides derived by an in-vitro selection process called SELEX (Systematic Evolution of Ligands by EXperimental enrichment), are important candidates for therapeutic and diagnostic applications. RNA aptamers have high affinity and specificity for their target molecules.

The research group designed a chimeric RNA aptamer against the Axl, an oncogenic tyrosine kinase receptor, which is overexpressed by most cancer cells. The aptamer Axl-148b displays high specificity to Axl positive cancer cells, ultimately inhibiting its …

2019年4月8日 · Here we describe the Tornado (Twister-optimized RNA for durable overexpression) expression system for achieving rapid RNA circularization, resulting in RNA aptamers with high stability and...

2022年9月13日 · RNA aptamers, also known as nucleic-acid monoclonal antibodies (mAbs), are artificial, single-stranded short RNAs that can bind proteins and biomolecules with high specificity and affinity. Instead of binding to the RISC, following cellular uptake, RNA aptamers assume a folded structure to interact with the disease-associated target directly.

One DNA aptamer (AS1411) and one L-form RNA aptamer (NOX-A12) have undergone clinical trials for the treatment of cancers. AS1411, formerly named ARGO100 (Antisoma), is a 26-nt G-quadruplex DNA oligonucleotide (which was not evolved by classic SELEX 204 ) and is the first aptamer in clinical trials for the treatment of human cancer.

2012年1月1日 · In this review, we describe methods for generating RNA aptamers (the SELEX technique and modified SELEX processes) and therapeutic applications for diseases such as neovascular age-related macular degeneration (AMD), inflammatory diseases, and obesity.

2025年1月13日 · RNA aptamers are short, single-stranded RNA molecules with the unique ability to bind specific targets, making them valuable tools in biotechnology and medicine.

Here we explore some of the evolution of the aptamer field and the insights it has provided for conceptualizing an RNA world, from its nascence to our current endeavor employing aptamers in human proteomics to discover biomarkers of health and disease.

2013年3月31日 · RNA aptamers have proven to be of high therapeutic and diagnostic value with recent FDA approval of the first aptamer drug and additional ones in the clinical pipelines. It has also been found to be a particularly useful tool for cell-type specific delivery of other RNA therapeutics like siRNA.