In chemistry, the sedimentation coefficient (s) of a particle characterizes its sedimentation (tendency to settle out of suspension) during centrifugation. It is defined as the ratio of a particle's sedimentation velocity to the applied acceleration causing the sedimentation.

Using specialized sample cells and modern analysis software, researchers can use sedimentation velocity to determine the homogeneity of a sample and define whether it undergoes concentration-dependent association reactions.

Basic Principles of Sedimentation: The rate of sedimentation is dependent upon the applied centrifugal field (G) being directed readily outwards; this is determined by the square of the angular velocity of the rotor (ω in radians s-1) and the radians (r, in centimeters) of the particle from the axis of the rotation, according to the equation

In sedimentation velocity (SV), the movement of solutes in high centrifu-gal fields is interpreted using hydrodynamic theory to define the size, shape, and interactions of macromolecules.

In sedimentation velocity (SV), the evolution of the sedimentation process is studied, whereas in sedimentation equilibrium (SE), the final equilibrium distribution is examined.

2015年1月1日 · Sedimentation velocity analytical ultracentrifugation is a fundamental hydrodynamic method that measures the rate of sedimentation of macromolecular species under the influence of a high centrifugal field.

A sedimentation velocity run is rather fast, making it the cheapest among all Analytical Ultracentrifugation experiments. In the neighboring figure, consecutive scans of a sedimentation velocity experiment are displayed. They show the sedimentation profiles of a sedimenting species at constant time intervals.

In sedimentation velocity experiment one observes the evolution of the concentration distribution as a function of both radius and time. Normally, relatively high speeds are chosen so that a boundary is formed between the solution of sedimenting macromolecule and …

‘Fringe number’ (the number of fringes that cross a horizontal axis) is proportional to the solute (macromolecule or particle) concentration. When the fringes are horizontal, the concentration is steady (~constant). When the fringes turn vertical, the macromolecule concentration is …

2018年6月30日 · Sedimentation velocity analytical ultracentrifugation is a very powerful tool for the study of macromolecules and nanoparticles, with regard to their size distribution, their shape or solution conformation, and sometimes their composition.