Apr 2, 2019 · UnaG is a basic (constitutively fluorescent) green fluorescent protein published in 2013, derived from Anguilla japonica. It has low acid sensitivity. It requires the cofactor bilirubin for fluorescence.

Jan 14, 2020 · We introduce UnaG as a green-to-dark photoswitching fluorescent protein capable of high-quality super-resolution imaging with photon numbers equivalent to the brightest photoswitchable...

Jun 20, 2013 · UnaG is a vertebrate fluorescent protein that produces green fluorescence upon binding to bilirubin. The development of an UnaG-based human bilirubin assay may have promising clinical utility.

We introduce UnaG as a green-to-dark photoswitching fluorescent protein capable of high-quality super-resolution imaging with photon numbers equivalent to the brightest photoswitchable red protein. UnaG only fluoresces upon binding of a fluorogenic metabolite, bilirubin, enabling UV-free reversible photoswitching with easily controllable ...

UnaG is a recently discovered ligand-induced fluorescent protein that utilizes bound bilirubin (BR) as its fluorophore. The fluorescence of the UnaG-BR complex (holoUnaG) compares in quantum efficiency to that of enhanced green fluorescent protein, ...

On heterologous expression in cell lines or mouse brain, UnaG produces oxygen-independent green fluorescence. Remarkably, UnaG fluorescence is triggered by an endogenous ligand, bilirubin, a membrane-permeable heme metabolite and clinical health biomarker...

Feb 12, 2018 · UnaG belongs to the fatty-acid-binding protein (FABP) family, and is a unique ligand-inducible fluorescent protein [1]. It binds unconjugated bilirubin (BR) non-covalently as a fluorogenic ligand with high specificity and affinity (Kd = 98 pM), and the resulting UnaG-BR complex (holoUnaG) emits green fluorescence (λmax = 527 nm, Φ f = 0.51) [1].

We introduced enhanced UnaG (eUnaG), a ligand-activatable fluorescent protein, for conventional and super-resolution imaging of subcellular structures in the mammalian cells. eUnaG is a V2L mutant of UnaG with twice brighter bulk fluorescence.

Jun 20, 2013 · Here, we report the cloning and characterization of UnaG, a fluorescent protein from Japanese eel. UnaG belongs to the fatty-acid-binding protein (FABP) family, and expression in eel is restricted to small-diameter muscle fibers.

Jun 20, 2013 · UnaG belongs to the fatty-acid-binding protein (FABP) family, and expression in eel is restricted to small-diameter muscle fibers. On heterologous expression in cell lines or mouse brain, UnaG produces oxygen-independent green fluorescence.