HATU (Hexafluorophosphate Azabenzotriazole Tetramethyl Uronium) is a reagent used in peptide coupling chemistry to generate an active ester from a carboxylic acid. HATU is used along with Hünig's base ( N,N -diisopropylethylamine), or triethylamine to form amide bonds.

The reaction involves conversion of the carboxylic acid group in P2 with 1.5 equiv. of TSTU, a uronium-based coupling reagent, 35 to produce an NHS-activated ester, which is stable against hydrolysis, but reactive with amines in aqueous solution.

2011年5月19日 · Carboxylic acid esters can be prepared in excellent yields at room temperature from an acid and either a phenol or an aliphatic alcohol using the peptide coupling reagents, TBTU, TATU, or COMU, in the presence of organic bases. Reactions using TBTU and TATU are faster but do not occur with tertiary alcohols.

2013年5月3日 · The approach used in this paper allows the calculation of the free energy profile and enable us to explain the mechanism and kinetics of the neutral hydrolysis of normal (methyl acetate) and activated (methyl trifluoroacetate) esters.

For years, the common coupling reagent HBTU (and later, the related HATU) were thought to exist as uronium salts (O form). However, X-ray crystallographic analysis revealed their true structure as guanidinium salts (N form).

2002年2月1日 · It involves the hydrolysis of DNA, fluorescence labeling of modified and unmodified nucleotides, micellar electrokinetic chromatography, and laser -induced-fluorescence detection

Carboxylic acid esters can be prepared in very good yields at room temperature from an acid and either a phenol or an aliphatic alcohol using the peptide coupling reagents TBTU, TATU, or COMU, in the presence of organic bases.

COMU is a third generation of uronium-type coupling reagent based on ethyl 2-cyano-2-(hydroxyimino)acetate (Oxyma) as well as a morpholino carbon skeleton. The presence of the morpholino group has a marked influence on the solubility, stability and reactivity of the reagent.

Ester hydrolysis refers to the reaction in which esters are broken down into their respective components through the addition of water molecules. This reaction is essential in various biological processes and chemical reactions.

Hydrolysis is a most important reaction of esters. Acidic hydrolysis of an ester gives a carboxylic acid and an alcohol. Basic hydrolysis of an ester gives a carboxylate salt and an alcohol. products obtained? the extent of reaction? What is saponification? Write an equation for the acid-catalyzed hydrolysis of ethyl acetate.