2019年9月24日 · Accumulating evidence indicates that endocytosis of nanoparticles generates autophagosomes, and their subsequent fusion with lysosomes leads to the digestion of their content 45.

HA peptide can destroy lysosome membrane, make siRNA/NLS escape lysosome, and enter the nucleus under the action of NLS, resulting in efficient gene silencing effect. This kind of cancer treatment strategy based on Apn nanocage shows high biocompatibility and unique lysosome escape property, which significantly improves the drug delivery and ...

2024年12月1日 · Enhancing the progress of nano-therapeutic lysosome escape has a key impact on improving the efficacy, specificity, and reducing side effects of gynecological cancer treatment. This strategic breakthrough addresses one of the most significant challenges in nanomedicine by ensuring that therapeutic drugs can reach intracellular targets without ...

2016年11月8日 · 而纳米载体的转运效率很大程度上取决于其逃逸溶酶体(lysosome)的能力，即纳米载体进入细胞后，经膜泡包被并传递进入溶酶体，成功逃离至细胞质，保护其负载物避免被溶酶体内的多种消化酶降解。

2010年10月1日 · As demonstrated by use of lysosomal probes, LNCs reverted lysosome integrity similarly to V-ATPase inhibitors and in a size-dependent fashion with best efficiency for small nanoparticles. When loaded with paclitaxel, smallest LNCs …

In this review, we describe several examples of prototypical synthetic reagents that are capable of endosomal escape and examine their mechanisms of action, their efficiencies, and effects on cells. Although these delivery systems are chemically distinct, some commonalities in how they interact with cellular membranes can be inferred.

2024年12月9日 · This review summarize the promising strategies that facilitate siRNA overcoming endosomal/lysosomal barriers, including promoting escape once endocytosis into the endosomes/lysosomes and bypassing lysosomes via endosome-Golgi-ER pathway or nonendocytosis pathway. We aim to provide novel insights for …

2024年7月1日 · We meticulously analyze the use of smart nanocarriers, exploring diverse mechanisms such as pore formation, proton sponge effects, membrane destabilization, photochemical disruption, and the strategic use of endosomal escape agents.

Despite extensive research, there is no consensus regarding the compartment of escape, the cause of the inefficient escape and are currently lacking a robust method to detect the escape. Here, we review the currently known mechanisms of endosomal escape and the available methods to study this process.

2024年3月12日 · We propose that the development of currently lacking robust, quantitative high-throughput techniques to study endosomal escape is timely and essential. A better understanding of this process will enable better RNA-LNP designs with improved efficiency to unlock new therapeutic modalities.